384 Well Plate Qpcr Protocol

Hard shell pcr plates represent an innovative concept in pcr plate design.

384 well plate qpcr protocol. Use the plate configuration box to determine sample number row a1 12 is used for duplicate samples of standards and a no template control ntc. Integrate this robotic plate handler with one or two cfx systems to maximize throughput while maintaining a compact footprint. The applied biosystems quantstudio 5 real time pcr system is designed for users who need superior performance maximum dye versatility and security options in a real time pcr system that is affordable and easy to use. In an excel spreadsheet qpcr plate template here plan the layout of your plate.

If using a 384 well plate load a 5 μl rxn. Unknowns should either be run as duplicates or triplicates. Contrast the abi 7900 high throughput capability using automatic loading of 384 well plates with the illumina eco instrument that supports a single 48 well plate. Many qpcr instruments have been designed to support a specific range of applications e g.

The most suitable instrument meets the needs of the research. For a 96 well load 10 μl or 20 μl. The optimized design and analysis software is ideal for both first time and exper. With common well matrixes and high or low profile designs that are compatible with thermal cyclers the pcr plates.

Proven applied biosystems reliability and accuracy. The assay will most likely be sybr green and the plate will be either 96 well or 384 well. The following assumes a 5 μl load in a 384 well plate. This is important with large plates.

Polypropylene pcr plates are compatible with most thermal cyclers and are ideal for high throughput screening thermocycler applications in preparation for real time polymerase chain reaction applications small segments of dna or rna are isolated and placed in pcr plates. Applied biosystems cat 4311971 using a 2x sybr green pcr mix applied biosystems. Select your detector from the list of existing detectors or create a new one. Enter data only into blue colored boxes.

Less diluted in future qpcr assays. The plate map is located on the left panel and setup information is located on the right panel. Hard shell 384 well skirted pcr plates. This hard shell prevents the distortion and shrinkage that may occur when regular single component polypropylene pcr plates are exposed to the high temperatures of thermal cycling or heat sealing bio rad offers a variety of hard shell 96 well and 384 well.

You will now see a blank plate document split into two panels. The skirt and deck are molded from a rigid thermostable polymer. The qpcr protocol given here is optimized for 10ul reactions in 384 well plate format plates. Full compatibility with any standard or fast cycling 384 or 96 well plates and taqman array micro fluidic cards and reagents makes high productivity real time pcr accessible to any size lab.